Regulation of Vascular Endothelial Growth Factor Signaling by miR-200b

• Published in: Molecules and cells 2011, 32(1), , pp.77-82
• Main Authors: Choi, Y.C., Kyung Hee University, Yongin, Republic of Korea, Yoon, S.N., Kyung Hee University, Yongin, Republic of Korea, Jeong, Y.S., Kyung Hee University, Yongin, Republic of Korea, Yoon, J.S., Kyung Hee University, Yongin, Republic of Korea, Baek, K.H., Kyung Hee University, Yongin, Republic of Korea
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; Angiogenesis Inhibitors - genetics; ARN; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Blotting, Western; Cell Biology; Cell Line, Tumor; Collagen; Drug Combinations; Endothelium, Vascular; Enzyme-Linked Immunosorbent Assay; Flt-1; Genes, Reporter; Humans; KDR; Laminin; Life Sciences; Luciferases - analysis; microRNA; MicroRNAs - genetics; miR-200b; Mitogen-Activated Protein Kinase 1 - genetics; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - genetics; Mitogen-Activated Protein Kinase 3 - metabolism; Neoplasms - genetics; Neoplasms - metabolism; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - metabolism; Phosphorylation - genetics; Plasmids; Proteoglycans; RNA; Signal Transduction - genetics; Transfection; Vascular Endothelial Growth Factor A - antagonists & inhibitors; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Vascular Endothelial Growth Factor Receptor-1 - antagonists & inhibitors; Vascular Endothelial Growth Factor Receptor-1 - genetics; Vascular Endothelial Growth Factor Receptor-1 - metabolism; Vascular Endothelial Growth Factor Receptor-2 - antagonists & inhibitors; Vascular Endothelial Growth Factor Receptor-2 - genetics; Vascular Endothelial Growth Factor Receptor-2 - metabolism; VEGF; VEGF signaling; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-011-1042-2

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3'-untranslated region (3'-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-induced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.