Crystal structure of malonyl CoA-Acyl carrier protein transacylase from Xanthomanous oryzae pv. oryzae and its proposed binding with ACP

• Published in: Molecules and cells 2012, 33(1), , pp.19-25
• Main Authors: Sampath Natarajan, Konkuk University, Seoul, Republic of Korea, Kim, J.K., Konkuk University, Seoul, Republic of Korea, Jung, T.K., Konkuk University, Seoul, Republic of Korea, Thanh Thi Ngoc Doan, Konkuk University, Seoul, Republic of Korea, Ho-Phuong-Thuy Ngo, Konkuk University, Seoul, Republic of Korea, Hong, M.K., Konkuk University, Seoul, Republic of Korea, Kim, S.H., Konkuk University, Seoul, Republic of Korea, Viet Pham Tan, Konkuk University, Seoul, Republic of Korea, Ahn, S.J., Konkuk University, Seoul, Republic of Korea, Lee, S.H., Myongji University, Yongin, Republic of Korea, Han, Y.S., Konkuk University, Seoul, Republic of Korea, Ahn, Y.J., Sangmyung University, Seoul, Republic of Korea, Kang, L.W., Konkuk University, Seoul, Republic of Korea
• Format: Article
• Language: English
• Subjects: acyl carrier protein; Acyl Carrier Protein - metabolism; Acyl-Carrier Protein S-Malonyltransferase - chemistry; Acyl-Carrier Protein S-Malonyltransferase - genetics; Acyl-Carrier Protein S-Malonyltransferase - metabolism; Amino Acid Sequence; ARROZ; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; computational docking; Crystallization; fatty acid synthesis; Life Sciences; Malonyl Coenzyme A - metabolism; Molecular Sequence Data; RICE; RIZ; Xanthomanous oryzae pv. oryzae; Xanthomonas - enzymology; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-012-2155-y

Xanthomonas oryzae pv. oryzae (Xoo) is a plant bacterial pathogen that causes bacterial blight (BB) disease, resulting in serious production losses of rice. The crystal structure of malonyl CoA-acyl carrier protein transacylase (XoMCAT), encoded by the gene fabD (Xoo0880) from Xoo, was determined at 2.3 angstrom resolution in complex with N-cyclohexyl-2-aminoethansulfonic acid. Malonyl CoA-acyl carrier protein transacylase transfers malonyl group from malonyl CoA to acyl carrier protein (ACP). The transacylation step is essential in fatty acid synthesis. Based on the rationale, XoMCAT has been considered as a target for antibacterial agents against BB. Protein-protein interaction between XoMCAT and ACP was also extensively investigated using computational docking, and the proposed model revealed that ACP bound to the cleft between two XoMCAT subdomains.