Rice OsACDR1 (Oryza sativa Accelerated Cell Death and Resistance 1) Is a Potential Positive Regulator of Fungal Disease Resistance

Rice Oryza sativa accelerated cell death and resistance 1 (OsACDRT) encodes a putative Raf-Iike mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathway...

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Bibliographic Details
Published in:Molecules and cells 2009, 28(5), , pp.431-439
Main Authors: Kim, Jung-A, Cho, Kyoungwon, Singh, Raksha, Jung, Young-Ho, Jeong, Seung-Hee, Kim, So-Hee, Lee, Jae-eun, Cho, Yoon-Seong, Agrawal, Ganesh K., Rakwal, Randeep, Tamogami, Shigeru, Kersten, Birgit, Jeon, Jong-Seong, An, Gynheung, Jwa, Nam-Soo
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
ARROZ
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Cell Biology
DISEASE RESISTANCE
LESION
LESIONES
LESIONS
Life Sciences
MAPKKK
ORGANISMOS PATOGENOS
OsACDR1
pathogen
PATHOGENS
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
RICE
RIZ
생물학
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Summary:Rice Oryza sativa accelerated cell death and resistance 1 (OsACDRT) encodes a putative Raf-Iike mitogen-activated protein kinase kinase kinase (MAPKKK). We had previously reported upregulation of the OsACDR1 transcript by a range of environmental stimuli involved in eliciting defense-related pathways. Here we apply biochemical, gain and loss-of- function approaches to characterize OsACDR1 function in rice. The OsACDR1 protein showed autophosphorylation and possessed kinase activity. Rice plants overexpressing OsACDR1 exhibited spontaneous hypersensitive response (HR)-like lesions on leaves, upregulation of defense-related marker genes and accumulation of phenolic compounds and secondary metabolites (phytoalexins). These transgenic plants also acquired enhanced resistance to a fungal pathogen (Magnaporthe grisea) and showed inhibition of appressorial penetration on the leaf surface. In contrast, loss-of- function and RNA silenced OsACDR1 rice mutant plants showed downregulation of defense-related marker genes expressions and susceptibility to M. grisea. Furthermore, transient expression of an OsACDR1: GFP fusion protein in rice protoplast and onion epidermal cells revealed its localization to the nucleus. These results indicate that OsACDRI1 plays an importantrole in the positive regulation of disease resistance in rice.
ISSN:1016-8478
0219-1032
DOI:10.1007/s10059-009-0161-5