Full-length enriched cDNA library construction from tissues related to energy metabolism in pigs

• Published in: Molecules and cells 2009, 28(6), , pp.529-536
• Main Authors: Lee, K.T., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Byun, M.J., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Lim, D.J., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Kang, K.S., Seoul National University, Seoul, Republic of Korea, Kim, N.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Oh, J.H., Korea Institute of Toxicology, Daejeon, Republic of Korea, Chung, C.S., Chungbuk National University, Cheongju, Republic of Korea, Park, H.S., National Institute of Animal Science, RDA, Suwon, Republic of Korea, Shin, Y.H., Insilicogen, Inc., Suwon, Republic of Korea, Kim, T.H., National Institute of Animal Science, RDA, Suwon, Republic of Korea
• Format: Article
• Language: English
• Subjects: Actins - genetics; Actins - metabolism; Adenine Nucleotide Translocator 3 - genetics; Adenine Nucleotide Translocator 3 - metabolism; Adipose Tissue - metabolism; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cells, Cultured; CERDO; CORPS GRAS; Cytoskeleton - genetics; Energy Metabolism - genetics; FATS; full-length cDNA; Gene Expression Profiling; Gene Library; gene ontology; GRASAS; Induced Pluripotent Stem Cells - metabolism; Induced Pluripotent Stem Cells - pathology; Life Sciences; Liver - metabolism; Mitochondria, Liver - genetics; Mitochondria, Liver - metabolism; PORCIN; Protein Biosynthesis - genetics; Sequence Alignment; SWINE; Swine - genetics; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-009-0147-3

Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5' ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.