Overexpression of GmAKR1, a stress-induced aldo/keto reductase from soybean, retards nodule development

• Published in: Molecules and cells 2009, 27(2), , pp.217-223
• Main Authors: Hur, Y.S. (Sookmyung Women's University, Seoul, Republic of Korea), Shin, K.H. (Sookmyung Women's University, Seoul, Republic of Korea), Kim, S.H. (Sookmyung Women's University, Seoul, Republic of Korea), Nam, K.H. (Sookmyung Women's University, Seoul, Republic of Korea), Lee, M.S. (Sookmyung Women's University, Seoul, Republic of Korea), Chun, J.Y. (Seegene Inc., Seoul, Republic of Korea), Cheon, C.I. (Sookmyung Women's University, Seoul, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: 2,4-Dichlorophenoxyacetic Acid - pharmacology; Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - isolation & purification; Alcohol Oxidoreductases - metabolism; Aldehyde Reductase; Aldo-Keto Reductases; Aldo/keto reductase; Amino Acid Sequence; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; AUXINAS; AUXINE; AUXINS; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cloning, Molecular; FORMATION DE NODOSITES; Glycine max - enzymology; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Herbicides - pharmacology; Life Sciences; Molecular Sequence Data; NODULACION; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - drug effects; Plant Roots - enzymology; Plant Roots - microbiology; Plants, Genetically Modified; promoter analysis; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Rhizobiaceae - pathogenicity; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Plant - genetics; RNA, Plant - metabolism; ROOT NODULATION; Root Nodules, Plant - drug effects; Root Nodules, Plant - enzymology; Root Nodules, Plant - microbiology; Seedlings - drug effects; Seedlings - enzymology; Seedlings - microbiology; Sequence Homology, Amino Acid; Symbiosis; transgenic hairy root; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-009-0027-x

Development of symbiotic root nodules in legumes involves the induction and repression of numerous genes in conjunction with changes in the level of phytohormones. We have isolated several genes that exhibit differential expression patterns during the development of soybean nodules. One of such genes, which were repressed in mature nodules, was identified as a putative aldo/keto reductase and thus named Glycine max aldo/keto reductase 1 (GmAKR1). GmAKR1 appears to be a close relative of a yeast aldo/keto reductase YakC whose in vivo substrate has not been identified yet. The expression of GmAKR1 in soybean showed a root-specific expression pattern and inducibility by a synthetic auxin analogue 2,4-D, which appeared to be corroborated by presence of the root-specific element and the stress-response element in the promoter region. In addition, constitutive overexpression of GmAKR1 in transgenic soybean hairy roots inhibited nodule development, which suggests that it plays a negative role in the regulation of nodule development. One of the Arabidopsis orthologues of GmAKR1 is the ARF-GAP domain 2 protein, which is a potential negative regulator of vesicle trafficking; therefore GmAKR1 may have a similar function in the roots and nodules of legume plants.