Sustained viral activity of epstein-barr virus contributes to cellular immortalization of lymphoblastoid cell lines

EBV-transformed lymphoblastoid cell lines (LCLs) are used as a resource for human genetic, immunological, and pharmacogenomic studies. We investigated the biological activity of 20 LCL strains during continuous long-term subculture up to a passage number of 160. Out of 20 LCL strains, 17 proliferate...

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Bibliographic Details
Published in:Molecules and cells 2009, 27(2), , pp.143-148
Main Authors: Jeon, J.P. (Korea Center for Disease Control and Prevention, Seoul, Republic of Korea), Nam, H.Y. (Korea Center for Disease Control and Prevention, Seoul, Republic of Korea), Shim, S.M. (Korea Center for Disease Control and Prevention, Seoul, Republic of Korea), Han, B.G. (Korea Center for Disease Control and Prevention, Seoul, Republic of Korea), E-mail: bokghee@yahoo.com
Format: Article
Language:English
Subjects:
Adult
Aged
B-Lymphocytes - pathology
B-Lymphocytes - virology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Cell Biology
Cell Transformation, Viral - physiology
CELLS
CELLULE
CELULAS
EBV
Epstein-Barr virus
Female
Herpesvirus 4, Human - physiology
Humans
immortalization
Life Sciences
lymphoblastoid cell line
Male
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Viral - genetics
Telomerase - metabolism
Telomere - physiology
생물학
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Summary:EBV-transformed lymphoblastoid cell lines (LCLs) are used as a resource for human genetic, immunological, and pharmacogenomic studies. We investigated the biological activity of 20 LCL strains during continuous long-term subculture up to a passage number of 160. Out of 20 LCL strains, 17 proliferated up to a passage number of 160, at which point LCLs are generally considered as "immortalized". The other three LCL strains lost the ability to proliferate at an average passage number of 41, during which these LCLs may have undergone cellular crisis. These non-immortal LCL strains exhibited no telomerase activity, decreased EBV gene expression, and a lower copy number of the EBV genome and mitochondrial DNA when compared with immortal LCLs. Thus, this study suggests that sustained EBV viral activity as well as telomerase activity may be required for complete LCL immortalization.
ISSN:1016-8478
0219-1032
DOI:10.1007/s10059-009-0018-y