Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera β-glucosidase genes

• Published in: Molecules and cells 2009, 28(4), , pp.369-373
• Main Authors: Jeon, Eugene, Hyeon, Jeong-eun, Suh, Dong Jin, Suh, Young-Woong, Kim, Seoung Wook, Song, Kwang Ho, Han, Sung Ok
• Format: Article
• Language: English
• Subjects: beta-Glucosidase - genetics; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cellulase - genetics; Cellulose - metabolism; Clostridium thermocellum - enzymology; Ethanol - chemical synthesis; Glucose - metabolism; Life Sciences; Organisms, Genetically Modified; Saccharomyces cerevisiae - genetics; Saccharomycopsis - enzymology; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-009-0131-y

Heterologous secretory expression of endoglucanase E ( Clostridium thermocellum ) and β-glucosidase 1 ( Saccharomycopsis fibuligera ) was achieved in Saccharomyces cerevisiae fermentation cultures as an α-mating factor signal peptide fusion, based on the native enzyme coding sequence. Ethanol production depends on simultaneous saccharification of cellulose to glucose and fermentation of glucose to ethanol by a recombinant yeast strain as a microbial biocatalyst. Recombinant yeast strain expressing endoglucanase and β-glucosidase was able to produce ethanol from β-glucan, CMC and acid swollen cellulose. This indicates that the resultant yeast strain of this study acts efficiently as a whole cell biocatalyst.