Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper

• Published in: Molecules and cells 2009, 27(3), , pp.329-336
• Main Authors: Hwang, J.N. (Seoul National University, Seoul, Republic of Korea), Li, J.J. (Seoul National University, Seoul, Republic of Korea), Liu, Wing-Yee (Seoul National University, Seoul, Republic of Korea), An, S.J. (Seoul National University, Seoul, Republic of Korea), Cho, H.J. (Plant Breeding and NongWoo Bio Ltd., Suwon, Republic of Korea), Her, N.H. (NongWoo Bio Ltd., Suwon, Republic of Korea), Yeam, I.H. (Cornell University, USA), Kim, D.S. (National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea), Kang, B.C. (Seoul National University, Seoul, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Capsicum - genetics; Capsicum - virology; Cell Biology; Chromosome Mapping; eIF4E; eIFiso4E; Eukaryotic Initiation Factor-4E - genetics; Gene Expression Regulation, Plant; Gene Expression Regulation, Viral; Genes, Recessive - genetics; Genetic Predisposition to Disease; host factor; Life Sciences; Mutation; PEPPER; PIMIENTA; Plant Diseases - genetics; Plant Diseases - virology; POIVRE; Polymerase Chain Reaction; Polymorphism, Genetic; Potyvirus - pathogenicity; recessive virus resistance; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-009-0042-y

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinai mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an eIF4E mutation (pvr1²) and C. annuum 'Perennial' containing an eIFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All F₁ plants showed resistance, and F₂ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five F₂ and 329 F₃ plants of 17 families were genotyped with pvr1² and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both pvr1² and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F₂ plants revealed that all plants containing homozygous genotypes of both pvr1² and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of eIF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.