Gamma-Irradiation and Doxorubicin Treatment of Normal Human Cells Cause Cell Cycle Arrest Via Different Pathways

• Published in: Molecules and cells 2005, 20(3), , pp.331-338
• Main Authors: Lee, S.M. (Korea University, Seoul, Republic of Korea), Youn, B.H. (Washington State University, Pullman, WA, USA), Kim, C.S. (Radiation and Health Research Institute, Seoul, Republic of Korea), Kim, C.S. (Korea University, Seoul, Republic of Korea), Kim, J. (Korea University, Seoul, Republic of Korea), E-mail: [e-mail], Kang, C.H. (Washington State University, Pullman, WA, USA)
• Format: Article
• Language: English
• Subjects: ANTIBIOTICOS; ANTIBIOTICS; Antibiotics, Antineoplastic - toxicity; ANTIBIOTIQUE; Biomarkers - metabolism; Cell Cycle - drug effects; Cell Cycle - radiation effects; Differential Gene Expression; DNA Damage - drug effects; DNA Damage - radiation effects; Doxorubicin - toxicity; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - radiation effects; GAMMA IRRADIATION; Gamma Rays; Gene Expression Profiling; Humans; IRRADIACION GAMMA; IRRADIATION GAMMA; Male; Normal Human Fibroblasts; Oligonucleotide Array Sequence Analysis; Signal Transduction - drug effects; Signal Transduction - radiation effects; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1016/S1016-8478(23)13235-3

Ionizing radiation and doxorubicin both produce oxidative damage and double-strand breaks in DNA. Double-strand breaks and oxidative damage are highly toxic and cause cell cycle arrest, provoking DNA repair and apoptosis in cancer cell lines. To investigate the response of normal human cells to agents causing oxidative damage, we monitored alterations in gene expression in F65 normal human fibroblasts. Treatment with γ-irradiation and doxorubicin altered the expression of 23 and 68 known genes, respectively, with no genes in common. Both agents altered the expression of genes involved in cell cycle arrest, and arrested the treated cells in G₂/M phase 12 h after treatment. 24 h after γ-irradiation, the percentage of G₁ cells increased, whereas after doxorubicin treatment the percentage of G₂/M cells remained constant for 24 h.