Attenuated Expression of Interferon-induced Protein Kinase PKR in a Simian Cell Devoid of Type Ⅰ Interferons

• Published in: Molecules and cells 2006, 21(1), , pp.21-28
• Main Authors: Park, S.H. (Korea University, Seoul, Republic of Korea), Choi, J.D. (Korea University, Seoul, Republic of Korea), Kang, J.I. (Korea University, Seoul, Republic of Korea), Choi, S.Y. (Korea University, Seoul, Republic of Korea), Hwang, S.B. (Hallym University, Chuncheon, Republic of Korea), Kim, J.S. (Korea University, Seoul, Republic of Korea), Ahn, B.Y. (Korea University, Seoul, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Adenoviruses, Simian - metabolism; Alternative Splicing; Amino Acid Sequence; Animals; Cell Line, Tumor; Chlorocebus aethiops; DNA, Complementary - genetics; eIF-2 Kinase - genetics; eIF-2 Kinase - metabolism; Exon 12 Deletion; Exons - genetics; Gene Expression Regulation - drug effects; Humans; Interferon alpha-2; Interferon Type I - deficiency; Interferon-alpha; Interferon-alpha - pharmacology; Molecular Sequence Data; Phosphorylation; Phosphotransferases - metabolism; PROTEINAS; PROTEINE; PROTEINS; Recombinant Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Deletion - genetics; Simian PKR; Tumor Cells, Cultured; Vero Cells; Vero E6 Cell; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1016/s1016-8478(23)12898-6

The interferon-induced, double-stranded RNA (dsRNA)-dependent protein kinase PKR plays a key role in interferon-mediated host defense against viral infection, and is implicated in cellular transformation and apoptosis. We have isolated a cDNA of simian PKR encoding a product with 83% amino acid identity to the human homolog and showed that PKR expression is significantly attenuated in the Vero E6 African green monkey kidney cells devoid of type Ⅰ interferon genes. A variant form of PKR lacking the exon 12 in the kinase domain is produced in these cells, presumably from an alternatively spliced transcript.