IL-4 Inhibits Cell Cycle Progression of Human Umbilical Vein Endothelial Cells by Affecting p53, p21Waf1, Cyclin D1, and Cyclin E Expression

IL-4 is emerging as a candidate cytokine for the treatment of inflammatory and autoimmune diseases. We have reported that IL-4 has anti-angiogenic activity and inhibits the growth of human umbilical vein endothelial cells (HUVEC) in response to vascular endothelial growth factor (VEGF) or fibroblast...

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Published in:Molecules and cells 2003, 16(1), , pp.92-96
Main Authors: Kim, Jinkoo, Cheon, In Su, Won, Yu-Jin, Kim, Young-Myeong, Choe, Jongseon
Format: Article
Language:English
Subjects:
생물학
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Summary:IL-4 is emerging as a candidate cytokine for the treatment of inflammatory and autoimmune diseases. We have reported that IL-4 has anti-angiogenic activity and inhibits the growth of human umbilical vein endothelial cells (HUVEC) in response to vascular endothelial growth factor (VEGF) or fibroblast growth factor-2 (FGF-2). Cell cycle analysis of this effect revealed that IL-4 arrests the growth of FGF-2- stimulated HUVEC in G0 + G1 phases. The absence of subdiploid cells showed that it did not induce apoptosis. Growth arrest was dose-dependent, but the percentage of G0 + G1 phase cells never exceeded 85%. An immunoblot analysis demonstrated that expression of p53 and p21Waf1 was increased and that of cyclin D1 and cyclin E decreased by IL-4. These results show that IL-4 inhibits endothelial cell growth by alte KCI Citation Count: 17
ISSN:1016-8478
0219-1032
DOI:10.1016/S1016-8478(23)13771-X