Dose-dependent UV Stabilization of p53 in Cultured Human Cells Undergoing Apoptosis Is Mediated by Poly(ADP-ribosyl)ation

• Published in: Molecules and cells 2006, 21(2), , pp.218-223
• Main Authors: Won, J.Y. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Chung, S.Y. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Kim, S.B. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Byun, B.H. (Daegu Haany University, Daegu, Republic of Korea), Yoon, Y.S. (Korea Institute of Oriental Medicine, Daejeon, Republic of Korea), Joe, C.O. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: APOPTOSE; APOPTOSIS; Apoptosis - physiology; Caspase 3 - metabolism; Caspase-3; Cells, Cultured - radiation effects; Dose-Response Relationship, Radiation; Enzyme Activation; Humans; p53; PARP; Poly (ADP-Ribose) Polymerase-1; Poly Adenosine Diphosphate Ribose - metabolism; Poly(ADP-ribose) Polymerases - metabolism; Proteasome; Proteasome Endopeptidase Complex - metabolism; Proteasome Inhibitors; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Ultraviolet Rays; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1016/s1016-8478(23)12883-4

The effect of poly(ADP-ribosyl)ation on the stability of p53 in SK-HEP1 cells treated with UV light was examined. Intracellular levels of p53 increased in cells treated with a low dose of UV light (20 J/㎡), whereas they increased but then declined after a higher dose of UV (100 J/㎡). Intracellular levels of p53 in the UV treated SK-HEP1 cells were dependent on the UV dose. Use of proteasome inhibitors revealed that p53 is degraded by proteasomal proteolysis after high doses of UV light.