Differential regulation of metallothionein-I and metallothionein-II mRNA expression in the rat brain following traumatic brain injury

In this study, we investigated the expression of metallothionein (MT)-I and MT-II in the rat brain following traumatic brain injury (TBI). In the early stage, significant induction of MT-I and MT-II were observed in various regions including ventricle walls, pia mater, and dentate gyrus. At 12-24 h...

Full description

Saved in:
Bibliographic Details
Published in:Molecules and cells 2004, 18(3), , pp.326-331
Main Authors: Kim, Eun Hae, Kim, Tae Sik, Sun, Woong, Kim, Dal Soo, Chung, Hyun-Soo, Kim, Dong Ki, Park, Sun-Hwa
Format: Article
Language:English
Subjects:
Animals
Brain - metabolism
Brain Injuries - metabolism
Cytokines - metabolism
Gene Expression Regulation
Immunohistochemistry
In Situ Hybridization
Male
Metallothionein - biosynthesis
Metallothionein - genetics
Microscopy, Fluorescence
Neuroglia - metabolism
Neurons - metabolism
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Time Factors
생물학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we investigated the expression of metallothionein (MT)-I and MT-II in the rat brain following traumatic brain injury (TBI). In the early stage, significant induction of MT-I and MT-II were observed in various regions including ventricle walls, pia mater, and dentate gyrus. At 12-24 h after TBI, strong induction of MT-I mRNA was observed in cerebral cortical layer II/III, amygdala, and piriform cortex where neurons reside. On the other hand, MT-II appeared to be expressed mainly in glial cells localized in the cerebral cortex and hippocampal formation. Three days after TBI, MTs were observed in the vimentin-positive astrocytes in the penumbra as revealed by double immunohistochemistry. The differences in expression of MT-I and MT-II in different brain regions and cell types (neuron vs. glial cells) suggests that multiple regulatory mechanisms are involved in the control of MT expression following brain injury.
ISSN:1016-8478
0219-1032
DOI:10.1016/S1016-8478(23)13120-7