Hepatic Fibrosis Inhibitory Effect of Peptides Isolated from Navicula incerta on TGF-β1 Induced Activation of LX-2 Human Hepatic Stellate Cells

• Published in: Preventive nutrition and food science 2013, 18(2), , pp.124-132
• Main Authors: Kang, K.H., Pukyong National University, Busan, Republic of Korea, Qian, Z.J., Chosun University, Jeonnam, Republic of Korea, Ryu, B.M., Pukyong National University, Busan, Republic of Korea, Karadeniz, F., Pukyong National University, Busan, Republic of Korea, Kim, D.K., Korea Basic Science Institute (KBSI), Jeju, Republic of Korea, Kim, S.K., Pukyong National University, Busan, Republic of Korea
• Format: Article
• Language: English
• Subjects: microalgae,hepatic stellate cells,hepatic fibrosis,liver injury; PEPTIDE; PEPTIDES; PEPTIDOS; 식품과학
• ISSN: 2287-1098; 2287-8602
• DOI: 10.3746/pnf.2013.18.2.124

In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-β1 activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-β1 stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-β1 induced LX-2 cells alleviated hepatic fibrosis. Moreover, α-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.