Enhanced gene delivery system using disulfide-linked chitosan immobilized with polyamidoamine

We developed a polyamidoamine (PAMAM) conjugated disulfide-linked chitosan (dsCS-PAM) for nonviral gene delivery. At weight ratio of 128, dsCS and dsCS-PAM showed positive zeta potential of around 9.5 mV and 14.5 mV, respectively, demonstrating their ability to form complexes with plasmid DNA (pDNA)...

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Bibliographic Details
Published in:Macromolecular research 2014, 22(4), , pp.370-376
Main Authors: Nam, Kihoon, Park, Ji Won, Bark, Heidi, Han, Jing, Nah, Jae-Woon, Jang, Mi-Kyeong, Kim, Sung Wan
Format: Article
Language:English
Subjects:
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Nanochemistry
Nanotechnology
Physical Chemistry
Polymer Sciences
Soft and Granular Matter
고분자공학
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Summary:We developed a polyamidoamine (PAMAM) conjugated disulfide-linked chitosan (dsCS-PAM) for nonviral gene delivery. At weight ratio of 128, dsCS and dsCS-PAM showed positive zeta potential of around 9.5 mV and 14.5 mV, respectively, demonstrating their ability to form complexes with plasmid DNA (pDNA) for cell transfection. CS-PAM and dsCS-PAM formed complexes with pDNA in a reducing environment containing 5 mM DLdithiothreitol (DTT) at weight ratio 32 and 16, respectively. PAMAM conjugated chitosan derivatives, dsCS-PAM and CS-PAM, showed higher in vitro transfection efficiency than unconjugated derivatives dsCS and CS. Approximately 80% of A549 and 293 cells were viable in the presence of up to 100 μg/mL of dsCS-PAM. The results of this study demonstrate that dsCS-PAM has the potential to be a safe gene delivery carrier.
ISSN:1598-5032
2092-7673
DOI:10.1007/s13233-014-2052-7