Development of novel photopolymerizable hyaluronic acid/heparin-based hydrogel scaffolds with a controlled release of growth factors for enhanced bone regeneration

In this study, the effect of novel BMP-2-loaded photo-cured hydrogels on in vitro and in vivo osteogenesis were explored. The well-defined HA-heparin hydrogel was prepared by a photopolymerization of aminoethyl methacrylate (AEMA) grafted hyaluronic acid (HA) and AEMA-grafted heparin. Subsequently,...

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Bibliographic Details
Published in:Macromolecular research 2016, 24(9), , pp.829-837
Main Authors: Bae, Min Soo, Ko, Na Re, Lee, Sang Jin, Lee, Jung Bok, Heo, Dong Nyoung, Byun, Wook, Choi, Byung-Joon, Jeon, Hoon Bong, Jang, Hee-Jae, Ahn, Ji-Yoon, Hwang, Deok-Sang, Jung, Bock-Young, Kwon, Il Keun
Format: Article
Language:English
Subjects:
Biocompatibility
Biological activity
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Controlled release
Growth factors
Heparin
Hyaluronic acid
Hydrogels
In vivo methods and tests
Mechanical properties
Nanochemistry
Nanotechnology
Photopolymerization
Physical Chemistry
Polymer Sciences
Regeneration (physiology)
Rheological properties
Rheology
Scaffolds
Soft and Granular Matter
Sustained release
Swelling ratio
고분자공학
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Summary:In this study, the effect of novel BMP-2-loaded photo-cured hydrogels on in vitro and in vivo osteogenesis were explored. The well-defined HA-heparin hydrogel was prepared by a photopolymerization of aminoethyl methacrylate (AEMA) grafted hyaluronic acid (HA) and AEMA-grafted heparin. Subsequently, the resulting hydrogels contained BMP-2 at different concentrations. First, a series of BMP-2-loaded hydrogels were characterized to determine their mechanical properties and release profiles. The results from rheology, swelling ratio, and release kinetics suggest that BMP-2 contained HA- g -Hhep gel (B/HA- g -Hhep gel) had sustained release during 4 weeks with minimal initial burst. Furthermore, the released BMP-2 maintained its biological activity. MTT and live/dead assays showed that the B/HA- g -Hhep gel had good biocompatibility to be used as a scaffold for bone regeneration. In vivo studies of B/HA- g -Hhep gel exhibited significantly enhanced osteogenesis, compared with other types of HA-heparin hydrogels. These excellent results indicated that B/HA- g -Hhep gel has a great potential for a controlled release of growth factor, leading to a good bone regeneration.
ISSN:1598-5032
2092-7673
DOI:10.1007/s13233-016-4112-7