Protein Binding Study of Isoflavone, Perillyl Alcohol and S-Ibuprofen by High-Performance Frontal Analysis

High-performance frontal analysis (HPFA) was used for a protein binding study of isoflavones (daidzein, genistin, and genistein), enantiomers of perillyl alcohol and S-ibuprofen to human serum albumin (HSA). The analyses were performed on a Develosil and Inertsil 100-Diol-5 column (10cm×4.6mm). Sodi...

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Bibliographic Details
Published in:The Korean journal of chemical engineering 2005, 22(3), 96, pp.465-469
Main Authors: 류경호, Du Young Choi, Long Mei Jin, Dexian Wang
Format: Article
Language:Korean
Subjects:
화학공학
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Summary:High-performance frontal analysis (HPFA) was used for a protein binding study of isoflavones (daidzein, genistin, and genistein), enantiomers of perillyl alcohol and S-ibuprofen to human serum albumin (HSA). The analyses were performed on a Develosil and Inertsil 100-Diol-5 column (10cm×4.6mm). Sodium phosphate solution (pH 7.4, ionic strength 0.17) was used as the mobile phase at a flow rate of 1 ml/min. To ensure the drug to be eluted as a trapezoidal peak with a plateau, injection volumes were each fixed up the zonal profile with an evident plateau appears. The unbound drug concentration was determined from a plateau height of the plateau region after that experimental data were fitted by Scatchard equation. The binding constants (K) and total binding affinities (nK) of drugs to HSA were calculated, respectively. KCI Citation Count: 1
ISSN:0256-1115
1975-7220