Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction

obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE celulose with glutaralde-hyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose wastreated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier....

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Bibliographic Details
Published in:The Korean journal of chemical engineering 2004, 21(1), 88, pp.201-205
Main Authors: Lian-Ying Zheng, Yu-Liang Xiao
Format: Article
Language:Korean
Subjects:
화학공학
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Summary:obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE celulose with glutaralde-hyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose wastreated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier. The optimaltemperature and pH was 60oC and 4.0, and the Km value was 18.87 g/L. Under optimal conditions, the activity of im-mobilized enzyme is 1.5 U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal tem-perature and Km value increased (from 50oC to 60oC, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was re-duced (from 5.0 to 4.0). The enzyme activity los was les than 20% after 10 times batch reaction; the imobilizedenzyme showed good operation stability. KCI Citation Count: 8
ISSN:0256-1115
1975-7220