Solid Extraction of Caffeine and Theophylline from Green Tea by Molecular Imprinted Polymers

• Published in: The Korean journal of chemical engineering 2004, 21(4), 91, pp.853-857
• Main Authors: 류경호, Dexian Wang, Seung Pyo Hong
• Format: Article
• Language: Korean
• Subjects: 화학공학
• ISSN: 0256-1115; 1975-7220

This paper involves a feasibility study on using molecular imprinted polymers as the sorbent materialsin solid phase extraction for cafeine and theophylline from green tea. Two kinds of MIPs, with cafeine-theophyllinemixture and pentoxifylline-theophylline mixture as the templates respectively, MAA as the monomer, EDMA as thecrosslinker and AIBN as the initiator, were applied to this purpose. Mixture solution of cafeine and theophylline (1mg/ml in acetonitrile) was applied to the solid extraction cartridges following a load, wash and elute procedure withacetonitrile, methanol, methanol-acetic acid (90/10, v/v) as the solvents, respectively. This solid phase extraction pro-tocol was applied for extraction of cafeine and theophylline from green tea. Comparison between the results obtainedwith the MIPs cartridges and a traditional C18 reversed-phase cartridge was made. It showed that the MIP-based sorbenton the solid phase extraction was comparable with that of C18 material. HPLC analysis using a C18 column (5mm, 2504.6 mm from Rstech corporation), methanol: water (60 : 40, v/v) as the mobile phase at a flow rate of 0.6 ml/min wasaplied for the quantitative determination. KCI Citation Count: 36