Lysobacter tyrosinelyticus sp. nov. isolated from Gyeryongsan national park soil

• Published in: The journal of microbiology 2015, 53(6), , pp.365-370
• Main Authors: Du, Juan, Singh, Hina, Ngo, Hien T. T, Won, KyungHwa, Kim, Ki-Young, Yi, Tae-Hoo
• Format: Article
• Language: English
• Subjects: Aerobiosis; alcohols; bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Cluster Analysis; College campuses; Cytosol - chemistry; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty acids; Fatty Acids - analysis; Genetic engineering; Gram-positive bacteria; Hydrogen-Ion Concentration; isoprenoids; Life Sciences; Lipids; Lysobacter; Lysobacter - classification; Lysobacter - genetics; Lysobacter - isolation & purification; Lysobacter - physiology; Microbiology; Molecular Sequence Data; Morphology; National parks; new species; nucleotide sequences; Parks, Recreational; phosphatidylethanolamines; Phospholipids - analysis; Phylogenetics; Phylogeny; Physiology; Quinones - analysis; Republic of Korea; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; Sodium chloride; Sodium Chloride - metabolism; soil; Soil Microbiology; Taxonomy; Temperature; ubiquinones; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-015-4729-9

A novel Gram-negative, rod-shaped (0.2–0.5 μm × 1.5–2.5 μm), aerobic, non-motile bacterium was isolated from Gyeryongsan national park soil, Republic of Korea. The novel isolate was designated as THG-DN8.2ᵀ. The strain grows optimally at 28°C, at pH 7 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel isolate shared the highest sequence similarity with Lysobacter oryzae KCTC 22249ᵀfollowed by Lysobacter yangpyeongensis KACC 11407ᵀand Lysobacter niabensis KACC 11587ᵀ. The DNA G+C content of strain THG-DN8.2T is 66.0 mol% and ubiquinone Q-8 is the main isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidyl-N-methylethanolamine. The major fatty acids of strain THG-DN8.2ᵀwere identified as iso-C₁₅:₀, iso-C₁₆:₀, and C₁₆:₁ω7с alcohol. The phylogenetic distinctiveness and phenotypic characteristics differentiated strain THG-DN8.2ᵀfrom closely related Lysobacter species. The results of polyphasic taxonomic analysis suggest that strain THG-DN8.2ᵀrepresents a novel species of the genus Lysobacter, for which the name Lysobacter tyrosinelyticus sp. nov. is proposed. The type strain is THG-DN8.2ᵀ(=KCTC 42235ᵀ=JCM 30320ᵀ).