MDA7/IL-24 is an anti-viral factor that inhibits influenza virus replication

Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus i...

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Bibliographic Details
Published in:The journal of microbiology 2016, 54(10), , pp.695-700
Main Authors: Seong, Rak-Kyun, Choi, Young-Ki, Shin, Ok Sarah
Format: Article
Language:English
Subjects:
2',5'-Oligoadenylate Synthetase - genetics
A549 Cells
Adjuvants, Immunologic - pharmacology
Antibiotics
Antigens
Antiviral Agents - pharmacology
Apoptosis
Biomedical and Life Sciences
Cancer
Cytokines
Gene Knockdown Techniques
Humans
Infections
Influenza
Influenza A virus
Influenza A virus - drug effects
Influenza A virus - physiology
Influenza, Human
Interferon
Interleukins - genetics
Interleukins - pharmacology
Kinases
Life Sciences
Melanoma
Microbiology
Myxovirus Resistance Proteins - genetics
Orthomyxoviridae
Penicillin
Proteins
Recombinant Proteins - pharmacology
Signal Transduction - genetics
Up-Regulation
Viral infections
Viral Nonstructural Proteins - genetics
Viral Plaque Assay
Virology
Virus Replication - drug effects
Viruses
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Summary:Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus infection are yet to be determined. In this study, we explored the anti-viral effect of recombinant IL-24 treatment during influenza infection. Infection of human lung adenocarcinoma cells (A549) with the influenza A virus up-regulated IL-24 mRNA and protein expression in a time-dependent manner. Pre-treatment of A549 cells with recombinant IL-24 protein effectively suppressed viral plaque formation. Furthermore, IL-24 treatment of A549 cells reduced viral non-structural protein 1 (NS1) synthesis, whereas IL-24 knockdown resulted in increased viral replication. Interestingly, IL-24 treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling. Taken together, our results suggest that IL-24 exerts a potent suppressive effect on influenza viral replication and can be used in the treatment of influenza infection.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-016-6383-2