Accumulation of Lipid Production in Chlorella minutissima by Triacylglycerol Biosynthesis-Related Genes Cloned from Saccharomyces cerevisiae and Yarrowia lipolytica

Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodies...

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Bibliographic Details
Published in:The journal of microbiology 2012, 50(3), , pp.526-534
Main Authors: Hsieh, Hsin-Ju, Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan, Su, Chia-Hung, Ming Chi University of Technology, New Taipei, Taiwan, Chien, Liang-Jung, Ming Chi University of Technology, New Taipei, Taiwan
Format: Article
Language:English
Subjects:
Algae
Alternative fuels
BIOCARBURANT
BIOCARBURANTE
Biodiesel fuels
BIOFUELS
Biomedical and Life Sciences
Biosynthesis
Biosynthetic Pathways - genetics
Chlorella - genetics
Chlorella - metabolism
Chlorella minutissima
Cloning, Molecular
diacylglycerol acyltransferase
DIESEL ENGINES
Life Sciences
Lipid Metabolism
Lipids
lysophosphatidic acid acyltransferase
Metabolic Engineering
microalgae
Microbiology
MOTEUR DIESEL
MOTORES DIESEL
phosphatidic acid phosphatase
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Triglycerides - biosynthesis
Yarrowia - enzymology
Yarrowia - genetics
Yarrowia lipolytica
Yeast
생물학
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Summary:Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-012-2041-5