Accumulation of Lipid Production in Chlorella minutissima by Triacylglycerol Biosynthesis-Related Genes Cloned from Saccharomyces cerevisiae and Yarrowia lipolytica

• Published in: The journal of microbiology 2012, 50(3), , pp.526-534
• Main Authors: Hsieh, Hsin-Ju, Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan, Su, Chia-Hung, Ming Chi University of Technology, New Taipei, Taiwan, Chien, Liang-Jung, Ming Chi University of Technology, New Taipei, Taiwan
• Format: Article
• Language: English
• Subjects: Algae; Alternative fuels; BIOCARBURANT; BIOCARBURANTE; Biodiesel fuels; BIOFUELS; Biomedical and Life Sciences; Biosynthesis; Biosynthetic Pathways - genetics; Chlorella - genetics; Chlorella - metabolism; Chlorella minutissima; Cloning, Molecular; diacylglycerol acyltransferase; DIESEL ENGINES; Life Sciences; Lipid Metabolism; Lipids; lysophosphatidic acid acyltransferase; Metabolic Engineering; microalgae; Microbiology; MOTEUR DIESEL; MOTORES DIESEL; phosphatidic acid phosphatase; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Triglycerides - biosynthesis; Yarrowia - enzymology; Yarrowia - genetics; Yarrowia lipolytica; Yeast; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-2041-5

Discovery of an alternative fuel is now an urgent matter because of the impending issue of oil depletion. Lipids synthesized in algal cells called triacylglycerols (TAGs) are thought to be of the most value as a potential biofuel source because they can use transesterification to manufacture biodiesel. Biodiesel is deemed as a good solution to overcoming the problem of oil depletion since it is capable of providing good performance similar to that of petroleum. Expression of several genomic sequences, including glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate acyltransferase, lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, and phospholipid:diacylglycerol acyltransferase, can be useful for manipulating metabolic pathways for biofuel production. In this study, we found this approach indeed increased the storage lipid content of C. minutissima UTEX 2219 up to 2-fold over that of wild type. Thus, we conclude this approach can be used with the biodiesel production platform of C. minutissima UTEX 2219 for high lipid production that will, in turn, enhance productivity.