Solid state production of polygalacturonase and xylanase by Trichoderma species using cantaloupe and watermelon rinds

• Published in: The journal of microbiology 2013, 51(5), , pp.605-611
• Main Authors: Mohamed, S.A., King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Al-Malki, A.L., King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Khan, J.A., King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Kabli, S.A., King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Al-Garni, S.M., King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
• Format: Article
• Language: English
• Subjects: Agriculture; Ammonium; Ammonium chloride; Ammonium nitrate; ammonium sulfate; bananas; Biomedical and Life Sciences; cantaloupes; Carbon; Carbon - metabolism; Citrullus - microbiology; Citrullus lanatus; Cucumis melo - microbiology; Enzymes; FERMENTACION EN ESTADO SOLIDO; Fermentation; FERMENTATION A L'ETAT SOLIDE; Fruits; Hydrogen-Ion Concentration; Lactose; Life Sciences; maltose; MELON; MELONS; Microbial Physiology and Biochemistry; Microbiology; Moisture content; Nitrates; Nitrogen; Nitrogen - metabolism; Nitrogen sources; PASTEQUE; polygalacturonase; Polygalacturonase - metabolism; rind; SANDIA; SOLID STATE FERMENTATION; starch; Sulfates; Temperature; TRICHODERMA; Trichoderma - enzymology; Trichoderma - growth & development; Trichoderma harzianum; Trichoderma virens; Urea; water content; WATERMELONS; xylanases; Xylosidases - metabolism; yeast extract; Yeasts; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-013-3016-x

Different solid state fermentation (SSF) sources were tested such as cantaloupe and watermelon rinds, orange and banana peels, for the production of polygalacturonase (PG) and xylanase (Xyl) by Trichoderma harzianum and Trichoderma virens. The maximum production of both PG and Xyl were obtained by T. harzianum and T. virnes grown on cantaloupe and watermelon rinds, respectively. Time course, moisture content, temperature, pH, supplementation with carbon and nitrogen sources were optimized to achieve the maximum production of both PG and Xyl of T. harzianum and T. virens using cantaloupe and watermelon rinds, respectively. The maximum production of PG and Xyl of T. harzianum and T. virens was recorded at 4'5 days of incubation, 50'66% moisture, temperature 28'35℃ and pH 6'7. The influence of supplementary carbon and nitrogen sources was studied. For T. harzianum, lactose enhanced PG activity from 87 to 120 units/g solid, where starch and maltose enhanced Xyl activity from 40 to 55'60 units/g solid for T. virnes. Among the nitrogen sources, ammonium sulphate, ammonium nitrate, yeast extract and urea increased PG activity from 90 to 110'113 units/g solid for T. harzianum. Similarly, ammonium chloride, ammonium sulphate and yeast extract increased Xyl activity from 45 to 55'70 units/g solid for T. virens.