Burkholderia denitrificans sp. nov., Isolated from the Soil of Dokdo Island, Korea

• Published in: The journal of microbiology 2012, 50(5), , pp.855-859
• Main Authors: Lee, C.M., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Weon, H.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Yoon, S.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Koo, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kwon, S.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
• Format: Article
• Language: English
• Subjects: 16S rRNA gene sequence; Base Composition; Biomedical and Life Sciences; Burkholderia; Burkholderia - classification; Burkholderia - genetics; Burkholderia - isolation & purification; Burkholderia - metabolism; Burkholderia denitrificans; Burkholderia sacchari; Data processing; Deoxyribonucleic acid; Diphosphatidylglycerol; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - metabolism; Forest soils; Forests; genomics; Islands; Life Sciences; Lipids; Microbiology; Molecular Sequence Data; New species; Nitrate; Nitrates; Nitrogen; PCB; pH effects; phosphatidylethanolamine; phosphatidylglycerol; Phylogenetics; Phylogeny; Polychlorinated biphenyls; Quinone; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sodium chloride; Software; Soil; Soil contamination; Soil Microbiology; TAXONOMIA; TAXONOMIE; TAXONOMY; Terpenes; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-1554-2

A novel, Gram-negative, bacterial strain KIS30-44∨T was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15-30℃, pH 5-9, 0-3% NaCl, and 950 mM KNO₃. KIS30-44∨T reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44∨T was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44∨T exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44∨T were C∧16:0, C∧18:1 ω7c and summed feature 3 (iso-C∧15:0 2-OH and C∧16:1 ω7c), and the strain contained half the amount of C∧17:0 cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44∨T represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44∨T =KACC 12733∨T =DSM 24336∨T) is proposed.