RNase G Participates in Processing of the 5'-end of 23S Ribosomal RNA

In Escherichia coli, primary rRNA transcripts must be processed by a complex process in which several ribonucleases are involved in order to generate mature 16S, 23S, and 5S rRNA molecules. While it is known that RNase G, a single-stranded RNA-specific endoribonuclease encoded by the rng gene, plays...

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Bibliographic Details
Published in:The journal of microbiology 2011, 49(3), , pp.508-511
Main Authors: Song, W.S., Chung-Ang University, Seoul, Republic of Korea, Lee, M.H., Chung-Ang University, Seoul, Republic of Korea, Lee, K.S., Chung-Ang University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
23S rRNA
5' Untranslated Regions - genetics
Base Sequence
Biomedical and Life Sciences
E coli
Endoribonucleases - genetics
Endoribonucleases - metabolism
ESCHERICHIA COLI
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Deletion
Life Sciences
Microbiology
Molecular Sequence Data
Nucleotide sequence
Nucleotides
Proteins
Ribonuclease
ribonuclease A
ribonuclease G
Ribonucleic acid
RNA
RNA Precursors - genetics
RNA Precursors - metabolism
RNA, Ribosomal, 23S - genetics
RNA, Ribosomal, 23S - metabolism
RNase G
Rng
rRNA
rRNA 16S
rRNA 23S
rRNA 5S
rRNA processing
생물학
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Summary:In Escherichia coli, primary rRNA transcripts must be processed by a complex process in which several ribonucleases are involved in order to generate mature 16S, 23S, and 5S rRNA molecules. While it is known that RNase G, a single-stranded RNA-specific endoribonuclease encoded by the rng gene, plays an active role in the maturation of the 5'-end of 16S rRNA, its involvement in the maturation of the 5'-end of 23S rRNA remains unclear. Here we show that E. coli cells deleted for the rng gene accumulate the 23S rRNA precursor containing an extra 77 nucleotides at its mature 5'-end. In vitro cleavage assays show that RNase G cleaves synthetic RNA containing a sequence encompassing the 5'-end to 77 nujcleotides upstream of mature 23S rRNA at two sites present in single-stranded regions. Our results suggest the involvement of RNase G in the processing of the 5'-region of 23S rRNA precursors.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-011-1198-7