Detection of Viruses in Farmed Rainbow Trout (Oncorhynchus mykiss) in Korea by RT-LAMP Assay

• Published in: The journal of microbiology 2011, 49(5), , pp.741-746
• Main Authors: Suebsing, Rungkarn, Gangneung-Wonju National University, Gangneung, Republic of Korea, Kim, J.H., Gangneung-Wonju National University, Gangneung, Republic of Korea, Kim, S.R., National Fisheries Research and Development Institute, Busan, Republic of Korea, Park, M.A., National Fisheries Research and Development Institute, Busan, Republic of Korea, Oh, M.J., Chonnam National University, Yeosu, Republic of Korea
• Format: Article
• Language: English
• Subjects: Animals; Aquaculture; Biomedical and Life Sciences; DNA polymerase; Fish; Fish diseases; Fish Diseases - virology; Genotype; Head Kidney - virology; IHNV; Infectious hematopoietic necrosis virus; Infectious hematopoietic necrosis virus - genetics; Infectious hematopoietic necrosis virus - isolation & purification; Infectious pancreatic necrosis virus; Infectious pancreatic necrosis virus - classification; Infectious pancreatic necrosis virus - genetics; Infectious pancreatic necrosis virus - isolation & purification; IPNV; Life Sciences; Microbiology; Novirhabdovirus - genetics; Novirhabdovirus - isolation & purification; Nucleic Acid Amplification Techniques; ONCORHYNCHUS MYKISS; Oncorhynchus mykiss - virology; Phylogeny; R&D; Republic of Korea; Research & development; RT-LAMP; Salmon; Sequence Analysis, DNA; Trout; VHSV; Viral diseases; Viral hemorrhagic septicemia virus; Virus Diseases - veterinary; Virus Diseases - virology; Viruses; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-011-1209-8

The viral diseases have been the serious problem in salmonid farming, and rainbow trout is not an exception. In this study, routine surveys were conducted for detecting of viruses in fanned rainbow trout (Oncorhynchus mykiss) in Korea during 2009-2010. Head kidneys from individual fish were employed for virus detection by using a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) were the target viruses in this study. 53.5% (46/86) were found to be IPNV-positive, while IHNV and VHSV showed RT-LAMP negative during examination for 2 years. Ten IPNV-positive samples were randomly selected for viral isolation and the cells showing CPEs were subjected to RT-LAMP, RT-PCR, and direct sequencing. Phylogenetic analysis showed that the rainbow trout isolate has high similarity homologies with the VR-299 strain, as previously described.