Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast

• Published in: The journal of microbiology 2010, 48(5), , pp.701-705
• Main Authors: Moon, D.G.R.M., Sungshin Women's University, Seoul, Republic of Korea, Park, Y.S., Sungshin Women's University, Seoul, Republic of Korea, Kim, C.Y., Sungshin Women's University, Seoul, Republic of Korea, Yoon, J.H., Sungshin Women's University, Seoul, Republic of Korea
• Format: Article
• Language: English
• Subjects: Active Transport, Cell Nucleus; Biomedical and Life Sciences; Colonies; Complementation; Cytoplasm; Genes; Genes, Essential; Genes, Fungal; Genetic Complementation Test; Genetic screening; Lethality; Life Sciences; Microbial Viability; Microbiology; mRNA; mRNA export; Mutants; Mutation; Nuclei; Proteins; Quality control; Retention; RNA polymerase; RNA, Messenger - metabolism; rsm1; Schizosaccharomyces - genetics; Schizosaccharomyces - growth & development; Schizosaccharomyces - metabolism; Schizosaccharomyces - physiology; SCHIZOSACCHAROMYCES POMBE; synthetic lethality; Vitamin B; Yeast; Yeasts; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-010-0353-x

To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rael, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.