Differential Expression Profiles of Alternaria alternate Genes in Response to Carbonyl Sulfide Fumigation

Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response...

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Bibliographic Details
Published in:The journal of microbiology 2010, 48(4), , pp.480-485
Main Authors: Liu, Tao, Chinese Academy of Inspection and Quarantine, Beijing, P. R. China, Li, Li, Chinese Academy of Inspection and Quarantine, Beijing, P. R. China, Wang, Yuejin, Chinese Academy of Inspection and Quarantine, Beijing, P. R. China, Zhan, Guoping, Chinese Academy of Inspection and Quarantine, Beijing, P. R. China, Liu, Bo, Chinese Academy of Inspection and Quarantine, Beijing, P. R. China
Format: Article
Language:English
Subjects:
Alternaria - drug effects
Alternaria - genetics
Alternaria - metabolism
ALTERNARIA ALTERNATA
Biomedical and Life Sciences
Carbonyl compounds
carbonyl sulfide
Cloning
Disease
expression profiles
Fumigation
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Fungicides, Industrial - pharmacology
Gene Expression Profiling
Gene Expression Regulation, Fungal - drug effects
Hybridization
Life Sciences
Microbiology
Ozone depletion
Pathogens
Plant Diseases - microbiology
Pyrus - microbiology
Signal transduction
Sulfides
Sulfur Oxides - pharmacology
suppression subtractive hybridization
Toxicity
생물학
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Summary:Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response to COS fumigation, we cloned 510 cDNA fragments of Alternaria alternata (Fr.) Keissler genes that are differentially expressed; these genes were cloned using suppression subtractive hybridization. Changes in the levels of transcripts of 79 fragments were confirmed by microarray analysis and qRT-PCR. Further homology search revealed that they are highly homologous to 41 genes of other fungi, which were related to general metabolism, growth and division, defense, cellular transport, and signal transduction. These results provide an overview of differential expression profiles of A. alternata genes following COS treatment and some new clues about the mechanism of COS fungitoxicity.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-010-9301-z