Nup211, the fission yeast homolog of Mlp1/Tpr, is involved in mRNA export

Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal muta...

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Bibliographic Details
Published in:The journal of microbiology 2009, 47(3), , pp.337-343
Main Authors: Bae, J.A., Sungshin Women's University, Seoul, Republic of Korea, Moon, D.G.R.M., Sungshin Women's University, Seoul, Republic of Korea, Yoon, J.H., Sungshin Women's University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Biomedical and Life Sciences
Fungal Proteins - genetics
Fungal Proteins - metabolism
Genes, Essential
Genes, Fungal
Genetic Complementation Test
Karyopherins - genetics
Karyopherins - metabolism
Life Sciences
Microbiology
mRNA export
nup211
Plasmids
Proteins
RNA, Fungal - metabolism
RNA, Messenger - metabolism
Schizosaccharomyces - genetics
Schizosaccharomyces - physiology
SCHIZOSACCHAROMYCES POMBE
Vitamin B
Yeast
Yeasts
생물학
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Summary:Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlp1/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-009-0125-7