Aliihoeflea aestuarii gen. nov., sp. nov., a Novel Bacterium Isolated from Tidal Flat Sediment

• Published in: The journal of microbiology 2008, 46(5), , pp.594-598
• Main Authors: Roh, S.W. (University of Science and Technology, Daejeon, Republic of Korea), Kim, K.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Nam, Y.D. (University of Science and Technology, Daejeon, Republic of Korea), Chang, H.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Kim, M.S. (University of Science and Technology, Daejeon, Republic of Korea), Shin, K.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Yoon, J.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Oh, H.M. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Bae, J.W. (University of Science and Technology, Daejeon, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Aliihoeflea aestuarii gen. nov., sp. nov; Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - metabolism; Biomedical and Life Sciences; Cloning; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty acids; Fatty Acids - metabolism; Geologic Sediments - microbiology; Life Sciences; Microbiology; Microscopy; Molecular Sequence Data; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; TAXONOMIA; TAXONOMIE; TAXONOMY; Tidal flats; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-008-0196-x

A novel Gram-negative and rod-shaped bacterium, designated N8∨T, was isolated from tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that N8∨T strain is associated with the family Phyllobacteriaceae: two uncultured clones (98.4 and 99.8% 16S rRNA gene sequence similarity) and the genus Mesorhizobium (less-than or equal to 97.0%). The novel strain formed a separate clade with uncultured clones in the phylogenetic tree based on 16S rRNA gene sequences. Cellular fatty acid profiles predominately comprised C∧18:1 ω7c and C∧19:0 cyclo ω8c. The major isoprenoid quinone is ubiquinone-10 and genomic DNA G+C content is 53.4 mol%. The polyphasic taxonomic study indicates that the novel strain N8T represents a novel species of the new genus in the family Phyllobacteriaceae, named Aliihoeflea aestuarii. The type strain is N8∨T (= KCTC 22052∨T= JCM 15118∨T= DSM 19536∨T).