Organization of three rRNA (rrn) operons from Sphingobium chungbukense DJ77

• Published in: The journal of microbiology 2008, 46(6), , pp.697-703
• Main Authors: Yeon, S.M. (Chungbuk National University, Cheongju, Republic of Korea), Choi, B.S. (Seoul National University, Seoul, Republic of Korea), Kim, Y.C. (Chungbuk National University, Cheongju, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: 16S rRNA; 23S rRNA; 5S rRNA; Alphaproteobacteria - genetics; ARN; Base Sequence; Biomedical and Life Sciences; Genomes; Life Sciences; Microbiology; Molecular Sequence Data; Phylogenetics; Phylogeny; RNA; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; RNA, Ribosomal, 5S - genetics; RNA, Transfer - genetics; rrn operons; rRNA Operon - genetics; Sequence Analysis, DNA; Sphingobium chungbukense DJ77; Sphingomonadaceae - genetics; Sphingomonas - classification; Sphingomonas - genetics; tRNA; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-008-0193-0

The nucleotide sequences of all three rRNA operons (rrnA, rrnB, and rrnC) of Sphingobium chungbukense DJ77 were determined. The three rrn operons have the same gene order (16S rRNA-tRNA∨Ile-tRNA∨Ala-23S rRNA-5S rRNA-tRNA∨fMet). The nucleotide sequences were identical over a 5,468 bp region spanning the 16S rRNA gene to the 5S rRNA gene. Variability was observed in the 5S rRNA-tRNA∨fMet spacer sequence of rrnB. The tRNA∨fMet gene sequences were identical except for two bases (T∧5794 and A∧5871 in rrnB, T∧5942 and A∧5956 in rrnA, but C∧5942 and G∧5956 in rrnC). Comparative sequence analyses of ribosomal RNA operons from DJ77 with those of the class Alphaproteobacteria, to which the genus Sphingobium belongs, reveal close evolutionary relationships with other members of the order Sphingomonadales.