Arthrobacter soli sp nov., a novel bacterium isolated from wastewater reservoir sediment

• Published in: The journal of microbiology 2008, 46(1), , pp.40-44
• Main Authors: Roh, S.W. (Korea University of Science and Technology, Daejeon, Republic of Korea), Sung, Y.B. (Environmental Research Department, Research Institute of Industrial Science and Technology, Gwangyang, Republic of Korea), Nam, Y.D. (Korea University of Science and Technology, Daejeon, Republic of Korea), Chang, H.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Kim, K.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Yoon, J.H. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Jeon, C.O. (Gyeongsang National University, Jinju, Republic of Korea), Oh, H.M. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), Bae, J.W. (Korea University of Science and Technology, Daejeon, Republic of Korea), E-mail: [e-mail]
• Format: Article
• Language: English
• Subjects: Arthrobacter; Arthrobacter - classification; Arthrobacter - genetics; Arthrobacter - isolation & purification; Arthrobacter - physiology; Arthrobacter protophormiae; Arthrobacter soli sp nov; Bacteria; Base Composition; Biochemical tests; Biomedical and Life Sciences; Chromatography; Deoxyribonucleic acid; DNA; DNA, Ribosomal - genetics; Fatty acids; Fatty Acids - chemistry; Genes, rRNA - genetics; Geologic Sediments - microbiology; Korea; Life Sciences; Microbiology; Micrococcaceae; Microorganisms; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogenetics; Phylogeny; Physiology; Reservoirs; RNA, Ribosomal, 16S - genetics; Sediments; Sequence Analysis, DNA; TAXONOMIA; TAXONOMIE; TAXONOMY; Waste Disposal, Fluid; wastewater reservoir sediment; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-007-0239-8

A novel Gram-positive bacterium, designated SYB2∨T, was isolated from wastewater reservoir sediment, and a polyphasic taxonomic study was conducted based on its morphological, physiological, and biochemical features, as well as the analysis of its 16S rRNA gene sequence. During the phylogenetic analysis of the strain SYB2∨T, results of a 16S rRNA gene sequence analysis placed this bacterium in the genus Arthrobacter within the family Hicrococcaceae. SYB2∨T and Arthrobacter protophormiae ATCC 19271∨T, the most closely related species, both exhibited a 16S rRNA gene sequence similarity of 98.99%. The genomic DNA G+C content of the novel strain was found to be 62.0 mol%. The predominant fatty acid composition was anteiso-C∧15:0, anteiso-C∧17:0, iso-C∧16:0, and iso-C∧15:0. Analysis of 16S rRNA gene sequences and DNA-DNA relatedness, as well as physiological and biochemical tests, showed genotypic and phenotypic differences between strain SYB2∨T and other Arthrobacter species. The type strain of the novel species was identified as SYB2∨T (= KCTC 19291∨T = DSM 19449∨T).