Diversity of endophytic enterobacteria associated with different host plants

• Published in: The journal of microbiology 2008, 46(4), , pp.373-379
• Main Authors: Torres, Adalgisa Ribeiro (University of Sao Paulo (ESALQ/USP), Piracicaba, Padua Dias, Brazil), Araujo, Welington Luiz (University of Sao Paulo (ESALQ/USP), Piracicaba, Padua Dias, Brazil), E-mail: [e-mail], Cursino, Luciana (University of Sao Paulo (ESALQ/USP), Piracicaba, Padua Dias, Brazil), Hungria, Mariangela (Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil), Plotegher, Fabio (Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil), Mostasso, Fabio Luis (Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil), Azevedo, Joao Lucio (University of Sao Paulo (ESALQ/USP), Piracicaba, Padua Dias, Brazil)
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; antibiotic susceptibility; Antibiotics; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biodiversity; Biomedical and Life Sciences; BOX-PCR; Cellulase; Cellulase - genetics; Cellulase - metabolism; cellulase production; Citrus; diversity; DNA Fingerprinting; DNA, Bacterial - genetics; ENDOFITAS; ENDOPHYTE; ENDOPHYTES; Enterobacter; Enterobacteriaceae - classification; Enterobacteriaceae - drug effects; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; Eucalyptus; Genetic diversity; Genotypes; Host plants; Life Sciences; Microbiology; Molecular Sequence Data; Pantoea agglomerans; Phenols; Phylogeny; Plant species; Plants - microbiology; RNA, Ribosomal, 16S - genetics; Soybeans; Sugarcane; Symbiosis; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-007-0165-9

Fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. Susceptibility was found on both tested antibiotics. However, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. Cellulase production also changed according to host plants. The diversity of these. isolates was estimated by employing BOX-PCR genomic fingerprints and 16S rDNA sequencing. In total, twenty-three distinct operational taxonomic units (OTUs) were identified by employing a criterion of 60% fingerprint similarity as a surrogate for an OTU. The 23 OTUs belong to the Pantoea and Enterobacter genera, while their high diversity could be an indication of paraphyletic classification. Isolates representing nine different OTUs belong to Pantoea agglomerans, P. ananatis, P. stewartii, Enterobacter sp., and E. homaechei. The results of this study suggest that plant species may select endophytic bacterial genotypes. It has also become apparent that a review of the Pantoea/Enterobacter genera may be necessary.