Comamonas granuli sp. nov., isolated from granules used in a wastewater treatment plant

• Published in: The journal of microbiology 2008, 46(4), , pp.390-395
• Main Authors: Kim, K.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Ten, Leonid N. (Paichai University, Daejeon, Republic of Korea), Liu, Qing-Mei (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Im, W.T. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: [e-mail], Lee, S.T. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea)
• Format: Article
• Language: English
• Subjects: 16S rRNA gene; Base Composition; betaproteobacteria; Biomedical and Life Sciences; Bioreactors - microbiology; Comamonas; Comamonas - classification; Comamonas - genetics; Comamonas - isolation & purification; Comamonas - metabolism; Comamonas granuli; Comamonas nitrativorans; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - metabolism; GENERA; GENRE; Industrial Waste; Life Sciences; Microbiology; Phylogenetics; Phylogeny; polyphasic taxonomy; RNA, Ribosomal, 16S - genetics; Sewage - microbiology; Wastewater treatment plants; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-008-0019-0

A Gram-negative, motile, rod-shaped, non-spore-forming bacterial strain, designated as Ko03∨T, was isolated from microbial granules, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. The isolate was positive for catalase and oxidase, but negative for gelatinase and β-galactosidase. Phylogenetic analyses using the 168 rRNA gene sequence showed that the strain formed a monophyletic branch towards the periphery of the evolutionary radiation occupied by the genus Comamonas, its closest neighbors being Comamonas koreensis KCTC 12005∨T (95.9% sequence similarity), Comamonas nitrativorans DSM 13191∨T, (95.7%), and Comamonas odontotermitis LMG 23579∨T (95.7%). Strain Ko03∨T had a genomic DNA G+C content of 68.4 mol% and the predominant respiratory quinone was Q-8. The major fatty acids were C∧16:1 ω7c (44.7%), C∧16:0 (28.1%), C∧18:1 (16.1%), and C∧10:0 3-OH (3.5%). These chemo-taxonomic results supported the affiliation of strain Ko03∨T to the genus Comamonas. However, low 168 rRNA gene sequence similarity values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain Ko03∨T from recognized Comamonas species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Ko03∨T represents a novel species of the genus Comamonas for which the name Comamonas granuli sp. nov. is proposed. The type strain is Ko03∨T (= KCTC 12199∨T = NBRC 101663∨T).