Developmental expression and oxidative stress induction of proteome of glutathione S-transferases in Aedes albopictus (Diptera: Culicidae)

• Published in: Journal of Asia-Pacific entomology 2016, 19(3), , pp.869-875
• Main Authors: Hamzah, Siti Nasuha, Alias, Zazali
• Format: Article
• Language: English
• Subjects: Aedes albopictus; Developmental stages; Glutathione S-transferase; Oxidative stress; 농수해양학
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2016.07.017

The combination of proteomic approach and affinity chromatography (GSTrap™ HP) has been used to identify and investigate the differential expression of GSTs under toxic challenge and during the course of development in Aedes albopictus. There were multiple isoforms of glutathione transferases (GSTs) expressed in the three developmental stages of Aedes albopictus. On a 2D gel map, the results indicate that there were 19 spots with a molecular mass in the range of 24–30kDa. Three types of GSTs were identified from the spots; namely GSTS1, GSTD1, and GST Theta. Spots identified as GSTS1 and GSTD1 were further designated as GSTS1–1 to GSTS1–5 and GSTD1–1 to GSTD1–13 respectively. Isoforms GSTD1–10, GSTD1–11, GSTD1–12, GSTD1–13, and GSTT were absent in the adult life stage. Isoenzymes GSTS1–3, GSTD1–2, and GSTD1–6 appeared as the major expressed isoforms in all the life stages. The effect of free radical generators such as hydrogen peroxide and paraquat on the expression of the GST isoforms in larvae mosquitoes was also investigated. Isoforms GSTD1–1, GSTD1–4, GSTS1–4, and GSTD1–12 had >1.5 fold increase when the larvae were challenged with both chemicals. GSTS1–3, GSTS1–4, GSTD1–1, GSTD1–2, GSTD1–3, GSTD1–6, and GSTD1–12 were significantly expressed (p1.5 fold change when the larvae were challenged with hydrogen peroxide. On the contrary, different GST isoforms were responsive towards paraquat challenge. Those isoforms were GSTS1–2, GSTS1–4, GSTD1–1, GSTD1–5, GSTD1–9, GSTD1–11, GSTD1–12, and GSTT (with >1.5 fold change). The purified larvae GSTs seemed to have limited substrate specificities with the ability to only conjugate 1-chloro-2,4-dinitrobenzene (CDNB) and 1,2-dichloro-4-nitrobenzene (DCNB). The GSTs have however shown that they were capable of detoxifying lipid peroxidation products and possessing glutathione peroxidase activities. [Display omitted] •There are multiple isoforms of GSTs in Aedes albopictus and belonged to several classes.•Several isoforms are developmentally expressed.•Many isoforms were demonstrated to response (induced expression) during oxidative stress.