Isolation and functional characterization of the Brassica napus cruciferin gene cru4 promoter

The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5′ deletion analysis in transgenic Arabidopsis. In the β-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly ac...

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Bibliographic Details
Published in:Applied biological chemistry 2014, 57(5), , pp.555-560
Main Authors: Roh, Kyung Hee, Choi, Soo Bok, Han-Chul, Kang, Jong-Bum, Kim, Kim Hyun Uk, Lee, Kyeong-Ryeol, Kim Sun Hee
Format: Article
Language:English
Subjects:
Brassica
Brassica napus
Embryos
Gene deletion
Globulins
Proteins
Rape plants
Seed coats
Seeds
Tata box
농학
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Summary:The 12S globulin protein cruciferin is main seed storage protein in Brassica napus. To gain a better understanding of the Bncru4 promoter function, we conducted the promoter 5′ deletion analysis in transgenic Arabidopsis. In the β-glucuronidase (GUS) expression assay, Bncru4 promoter was strongly active in transgenic seeds. In addition, deletion of RY-elements (−236 bp region) dramatically decreased the promoter activity in seed embryos; however, the GUS expression could be observed in seed coat. Further deletion up to −113 bp region (removed up to the CAAT and TATA box), GUS expression was completely abolished in all tissues. These results were consistent with that of the GUS activity in transgenic seeds. Therefore, we consider that RYelement is crucial to the seed-specific expression of Bncru4 promoter
ISSN:2468-0834
2468-0842
DOI:10.1007/s13765-014-4149-2