Pinoresinol diglucoside is screened as a putative α-glucosidase inhibiting compound in Actinidia arguta leaves

• Published in: Applied biological chemistry 2014, 57(4), , pp.473-479
• Main Authors: Kwon Dodan, Kim Gyoung Deuck, Kang Wonseok, Park, Jeong-Eun, Kim, Song Hee, Choe Eunok, Kim, Jung-In, Auh Joong-Hyuck
• Format: Article
• Language: English
• Subjects: Acarbose; Actinidia arguta; Diabetes mellitus; Ethanol; Fractionation; Glucosidase; Glycosides; Inhibitors; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolomics; Polyphenols; α-Glucosidase; 농학
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1007/s13765-014-4167-0

Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the α-glucosidase inhibitory compounds in A. arguta were screened and identified through α-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting α-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as α-glucosidase inhibitory agents, pinoresinol diglucoside was proposed to be a putative key compound for α-glucosidase inhibition in A. arguta. This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural α-glucosidase inhibitor.