Establishment of a simple method for purification of high purity chlorogenic acid from immature fruit of pear (Pyrus pyrifolia Nakai)

Chlorogenic acid [3-(3,4-dihydroxycinnamoyl)quinic acid, CGA] is one of the main compounds contained in immature pear fruits. In the present study, we established simple purification of CGA in high purity from the immature fruit of pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) which contains CGA in h...

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Bibliographic Details
Published in:Applied biological chemistry 2015, 58(3), , pp.335-341
Main Authors: Lee, Sang Won, Lee Yu Geon, Jeong-Yong, Cho, Kim Young Chool, Sang-Hyun, Lee, Kim Wol-Soo, Jae-Hak, Moon
Format: Article
Language:English
Subjects:
Acetic acid
Chlorogenic acid
Column chromatography
Ethyl acetate
Fruits
High performance liquid chromatography
Liquid chromatography
Purification
Purity
Pyrus pyrifolia
Quinic acid
농학
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Summary:Chlorogenic acid [3-(3,4-dihydroxycinnamoyl)quinic acid, CGA] is one of the main compounds contained in immature pear fruits. In the present study, we established simple purification of CGA in high purity from the immature fruit of pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) which contains CGA in high concentration. The 80 % MeOH extract (1.4 g) of immature pear fruits (20 g fresh wt.) was partitioned with ethyl acetate (EtOAc) under acidic condition (pH 2.65). Then, the EtOAc-soluble acidic layer (110.3 mg) was further purified using Diaion HP-20 column chromatography (H2O/MeOH, step wise). Then, the CGA-contained fractions were finally purified by preparative ODS-HPLC. The isolated CGA (24.3 mg) showed high purity (>99 %) and the total recovery was estimated to be 63.9 %.
ISSN:2468-0834
2468-0842
DOI:10.1007/s13765-015-0041-y