Gene Inactivation Study on gntK, a Putative C-methyltransferase Gene in Gentamicin Biosynthesis from Micromonospora echinospora

GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on t...

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Bibliographic Details
Published in:Applied biological chemistry 2012, 55(3), , pp.439-442
Main Authors: Karki, Suman, Myongji University, Yongin, Republic of Korea, Kim, J.Y., Myongji University, Yongin, Republic of Korea, Park, S.H., Mokpo National University, Muan, Republic of Korea, Kwon, H.J., Myongji University, Yongin, Republic of Korea
Format: Article
Language:English
Subjects:
Adenosylmethionine
Applied Microbiology
Biological Techniques
Bioorganic Chemistry
Biosynthesis
Chemistry
Chemistry and Materials Science
Deactivation
Domains
GENE
GENES
Gentamicin
gentamicin biosynthesis
gntK
Inactivation
Isomers
Methylation
Methyltransferase
Micromonospora echinospora
S-Adenosylmethionine
Short Communication
Vitamin B12
농학
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Summary:GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on these results, we propose that GntK is involved in Cmethylation on C-6' in gentamicin X2 but is dispensable in gentamicin biosynthesis.
ISSN:1738-2203
2468-0834
2234-344X
2468-0842
DOI:10.1007/s13765-012-2041-5