Identification of MicroRNAs and Their Putative Targets that Respond to Drought Stress in Solanum tuberosum

MicroRNAs (miRNAs) are small noncoding RNAs that are found in both animals and plants. miRNAs function as negative post-transcriptional regulators during plant development and in response to biotic and abiotic stresses. In present study, high-throughput DNA sequencing and alignments with sequences f...

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Bibliographic Details
Published in:Applied biological chemistry 2011, 54(3), , pp.317-324
Main Authors: Hwang, E.W., Sunmoon University, Asan, Republic of Korea, Shin, S.J., Sunmoon University, Asan, Republic of Korea, Kwon, H.B., Sunmoon University, Asan, Republic of Korea
Format: Article
Language:English
Subjects:
Applied Microbiology
Biological Techniques
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
deep sequencing
DNA sequencing
Drought
DROUGHT STRESS
ESTRES DE SEQUIA
Gene expression
MicroRNAs
miRNA
Nucleotide sequence
PAPA
POMME DE TERRE
Post-transcription
POTATOES
Regulators
Ribonucleic acid
RNA
Solanum tuberosum
STRESS DU A LA SECHERESSE
농학
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Summary:MicroRNAs (miRNAs) are small noncoding RNAs that are found in both animals and plants. miRNAs function as negative post-transcriptional regulators during plant development and in response to biotic and abiotic stresses. In present study, high-throughput DNA sequencing and alignments with sequences from microRNA database (miRBase), a microRNA database, were used to identify drought stress-related miRNAs. This method is useful for identifying miRNAs and determining their expression profiles when genomic sequences are not available. Three drought-induced miRNAs, stu-miR396, stu-miR156a, and stu-miR157a were identified in potato (Solanum tuberosum). Effects of drought stress on their expression profiles were demonstrated, and their target mRNAs were predicted.
ISSN:1738-2203
2468-0834
2234-344X
2468-0842
DOI:10.3839/jksabc.2011.051