Selection and Characterization of the Bacteriocin-Producing Bacterium, Bacillus subtilis BP6 Isolated from Chicken Gut against Salmonella gallinarum Causing Fowl-typhus

• Published in: Applied biological chemistry 2009, 52(1), , pp.80-87
• Main Authors: Jong-Hui, Lim, Sang-Dal, Kim
• Format: Article
• Language: English
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Aquatic birds; Bacillus subtilis; Bacteria; Bacteriocins; Biodegradation; Butanol; Cell culture; Cell walls; Chickens; E coli; Electrophoresis; Endopeptidase K; Fowl typhoid; Gel electrophoresis; Listeria; Listeria monocytogenes; Microorganisms; Mode of action; Molecular weight; Polyacrylamide; Poultry; Probiotics; Proteinase; rRNA 16S; Salmonella; Sequences; Sodium dodecyl sulfate; Sodium lauryl sulfate; Strains (organisms); Thermal stability; Trypsin; Typhoid; Typhus; Waterborne diseases; α-Amylase; 농학
• ISSN: 2468-0834; 2468-0842
• DOI: 10.3839/jksabc.2009.014

To select the antagonistic microorganisms against Salmonella gallinarum causing fowl typhoid, 31 strains were isolated from the gut of Korean native chicken. Among these, six strains could inhibit the growth of S. gallinarum. Strain BP6, which had strong inhibition activity against S. gallinarum, was identified as Bacillus subtilis BP6 by Biolog system and 16s rDNA sequencing. B. subtilis BP6 had antimicrobial activity toward various food-born pathogen such as Micrococcus luteus, B. cereus, Vibrio parahaemoliticus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O-157. The purified bacteriocin BP6 showed a single band of 20.7 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Furthermore, low (10 kDa) substances from culture supernatant also inhibited the growth of S. gallinarum simultaneously. When the purified bacteriocin BP6 and n-butanol extract of non-peptide antibiotic-like substances were treated with protease, proteinase K, and trypsin, the antimicrobial activity of purified bacteriocin BP6 was lost, whereas that of n-butanol extract was not. The antimicrobial activity of purified bacteriocin BP6, however, was not affected by the α-amylase treatment. The bacteriocin BP6 was heat-stable and had a bacteriolytic mode of action resulting in the degradation of the S. gallinarum cell wall, suggesting the bacteriocin-producing bacterium B. subtilis BP6 could be a non-antibiotic probiotic for prevention of chicken or duck typhus.