LPS를 처리한 RAW 264.7 세포에서 털여뀌와 양지꽃 추출물의 NF-κB 활성화 및 Nitric Oxide 생성 저해

• Published in: Journal of applied biological chemistry 2014, 57(3), , pp.205-210
• Main Authors: 최재훈, Je Hun Choi, 이승은, Seung Eun Lee, 이정훈, Jeong Hoon Lee, 김금숙, Geum Sook Kim, 노형준, Hyung Jun Noh, 김승유, Seung Yu Kim
• Format: Article
• Language: Korean
• Subjects: inflammation; nuclear factor-kappa B; Persicaria orientalis; Potentilla fragarioides; reactive oxygen species; 농학
• ISSN: 1976-0442; 2234-7941

Persicaria orientalis (L.) Spach (Po)와 Potentilla fragarioidesvar. major Maxim (Pf)의 추출물로 reactive oxygen species(ROS)와 같은 free radical의 억제를 통한 항염증 활성을 살펴보았다. 또한, Po와 Pf로 LPS를 처리한 murine macrophage RAW 264.7 세포에서 발생한 nitric oxide의 저해와 NF-κB의 핵으로 translocation의 저해를 살펴보았다. 3-morpholinosydnoniminehydrochloride (SIN-1) 50 μM에 의해 유도된 ROS의 Po에 의한 50% 저해값 (IC50)은 23.35±1.27 mg/mL, Pf에 의한 IC50은 8.46±1.22 mg/mL이었다. 또한, SIN-1 50 μM에 의해 유도된 peroxynitrite의 Po에 의한 IC50은 2.19±0.04 mg/mL, Pf에 의한 IC50은 0.80±0.02 mg/mL이었다. LPS 1 mg/mL을 처리한 RAW264.7 세포에서 Nitric oxide는 증가하였으나 Po와 Pf 추출물을 처리한 그룹에서 농도의존적, 유의적으로 감소하였다. Po추출물을 처리한 그룹의 Nitric oxide 생성량은 13.34±0.67 μM, Pf 추출물을 처리한 그룹의 Nitric oxide 생성량은 11.45±0.57μM이었다. 또한, Po와 Pf 추출물은 LPS를 처리한 RAW 264.7세포에서 NF-κB의 핵으로의 translocation을 저해하였다. 그러므로, Po와 Pf는 항염증 소재로서의 가능성이 충분하다고 사료된다. Persicaria orientalis (L.) Spach (Po) and Potentilla fragarioides var. major Maxim (Pf) extracts were analyzed to investigate anti-inflammation through their suppressing effects on free radicals such as reactive oxygen species (ROS). In addition, with regard to Po and Pf, an analysis was conducted of their inhibitory effect on nitric oxide, which is produced in lipopolysaccharide (LPS)-treated murine macrophage RAW 264.7 cells, and their inhibitory effect on the translocation of the nucleus of nuclear factor-kappa B (NF-κB). The IC50 value of ROS, which was induced by 50 μM 3-morpholinosydnonimine hydrochloride (SIN-1), was found to be 23.35±1.27 μg/mL due to the effect of the Po extract, and 8.46±1.22 μg/mL due to the effect of the Pf extract. In addition, the IC50 value of peroxynitrite treated with the Po extract was 2.19±0.04 μg/mL, whereas that of peroxynitrite treated with the Pf extract was 0.80±0.02 μg/mL. ROS and peroxynitrite were induced by 50 μM 3-morpholinosydnonimine hydrochloride. There was an increase in the amount of nitric oxide in the RAW 264.7 cells treated with LPS (1 μg/mL), whereas the level of NO was observed to significantly and dose-dependently decrease in the cells treated with Po and Pf. The amount of nitric oxide produced by the group treated with 10 μg/mL of the Pf extract was 11.45±0.57 μM. Furthermore, the Po extracts inhibited the translocation of the nucleus of NF-κB in LPS-treated RAW 264.7 cells. Therefore, it is highly possible that Po and Pf have anti-inflammatory properties.