Inhibition of Low Density Lipoprotein-oxidation, ACAT-1, and ACAT-2 by Lignans from the Bark of Machilus thunbergii

The bark of Machilus thunbergii was extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), butanol (n-BuOH), and H₂O, successively. From the EtOAc fraction, five lignans were isolated through the repeated silica gel, octadecyl silica gel...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied biological chemistry 2011, 54(1), , pp.63-66
Main Authors: Shrestha, Sabina, Kyung Hee University, Yongin, Republic of Korea, Park, J.H., Kyung Hee University, Yongin, Republic of Korea, Lee, D.Y., Kyung Hee University, Yongin, Republic of Korea, Cho, J.G., Kyung Hee University, Yongin, Republic of Korea, Lee, D.G., Kyung Hee University, Yongin, Republic of Korea, Cho, M.H., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Jeong, T.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Kang, H.C., Green Flower Cosmetics Co., Suwon, Republic of Korea, Baek, N.I., Kyung Hee University, Yongin, Republic of Korea
Format: Article
Language:English
Subjects:
ACAT-1
ACAT-2
LDL-oxidation
licarin A
LIGNANAS
LIGNANE
LIGNANS
machilin A
machilin F
Machilus thunbergii
nectandrin A
nectandrin B
농학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The bark of Machilus thunbergii was extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), butanol (n-BuOH), and H₂O, successively. From the EtOAc fraction, five lignans were isolated through the repeated silica gel, octadecyl silica gel (ODS) and, Sephadex LH-20 column chromatography. Based on nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared spectroscopy (IR) spectroscopic data, the chemical structures of the compounds were determined to be machilin A (1), machilin F (2), licarin A (3), nectandrin A (4), and nectandrin B, (5). This study presents comparative account of five lignans from M. thunbergii bark contributing inhibition of low density lipoprotein (LDL), ACAT-1, and ACAT-2. Compounds 2-5 showed varied degree of antioxidant activity on LDL with IC∧50 values of 2.1, 11.8, 15.3, and 4.1 μM. Compounds 1, 2, and 3 showed inhibition activity on ACAT-1 with values 63.4±6.9% (IC∧50=66.8 μM), 53.7±0.9% (IC∧50=109.2 μM), and 78.7±0.2% (IC∧50=40.6 μM), respectively, at a concentration of 50 mg/mL, and on ACAT-2 with values 47.3±1.5% (IC∧50=149.7 μM), 39.2±0.2% (IC∧50=165.2 μM), and 52.1±1.0% (IC∧50=131.0 μM), respectively, at a concentration of 50 mg/mL.
ISSN:1976-0442
2234-7941
DOI:10.3839/jabc.2011.011