Effects of 17β-Estradiol on the Plasminogen Activator System in Vascular Smooth Muscle Cells Treated with Lysophophatidylcholine

• Published in: Journal of menopausal medicine 2020, 26(1), , pp.9-17
• Main Authors: Yoon, Byung Koo, Kang, Young Hee, Oh, Won Jong, Lee, Dong Yun, Kim, Duk Kyung, Kessel, Bruce, Kang, Chi Dug
• Format: Article
• Language: English
• Subjects: Original; 산부인과학
• ISSN: 2288-6478; 2288-6761
• DOI: 10.6118/jmm.19005

When administered soon after menopause, hormone therapy can prevent coronary heart diseases in women. To explore the mechanism underlying the cardioprotective actions of estrogen, we investigated the effects of 17β-estradiol (17β-E₂) on the plasminogen activator system using cultured vascular smooth muscle cells (VSMCs). VSMCs were isolated from rat aortas. Protein expression of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were evaluated using Western blotting and enzyme-linked immunosorbent assay, respectively. The enzyme activity of PAI-1 in a conditioned medium was assessed via reverse fibrin overlay zymography and that of t-PA was assessed via fibrin overlay zymography. Gene expression was quantified using real-time reverse transcription-polymerase chain reaction. Following pre-treatment for 24 hours, 17β-E₂ suppressed both protein expression and enzyme activity of PAI-1 stimulated by lysophosphatidylcholine (lysoPC) in a significant and dose-dependent manner at a near physiological concentration. Moreover, 17β-E₂ (10⁻⁷ M) inhibited PAI-1 gene expression, and ICI 182,780-a specific estrogen receptor antagonist-blocked the effects of 17β-E₂ on the PAI-1 protein. 17β-E₂ did not affect t-PA secretion but significantly enhanced free t-PA activity through reduced binding to PAI-1. Furthermore, 17β-E₂ suppressed intracellular reactive oxygen species production and nuclear factor-κB-mediated transcription. In VSMCs stimulated with lysoPC, 17β-E₂ reduced PAI-1 expression through a non-receptor-mediated mechanism via antioxidant activity as well as a receptor-mediated mechanism; however, it did not alter t-PA secretion. Of note, 17β-E₂ suppressed PAI-1 activity and concurrently enhanced t-PA activity, suggesting a beneficial influence on fibrinolysis.