Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization

Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteo...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2009, 52(1), , pp.41-48
Main Authors: Kim, B.G., Konkuk University, Seoul, Republic of Korea, Kim, N.Y., Konkuk University, Seoul, Republic of Korea, Kim, J.H., Konkuk University, Seoul, Republic of Korea, Akimitsu, Kazuya, Kagawa University, Kagawa, Japan, Chong, Y.H., Konkuk University, Seoul, Republic of Korea, Ahn, J.H., Konkuk University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
Binding sites
Biomedical and Life Sciences
Biosynthesis
Chromatography
Cloning
E coli
Flavonoid O-diglucoside
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
GLICOSILTRANSFERASAS
Glucose
GLYCOSYLTRANSFERASE
GLYCOSYLTRANSFERASES
Hydroxyl groups
Kaempferol
Life Sciences
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Microorganisms
Molecular docking
Original Research
ORYZA SATIVA
Phylogenetics
Phylogeny
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Proteins
Quercetin
Reaction products
Rice
Substrates
UGT
Uridine
생물학
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Description
Summary:Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice.
ISSN:1226-9239
1867-0725
DOI:10.1007/s12374-008-9002-x