Expression Profiles of Class A Rice Heat Shock Transcription Factor Genes Under Abiotic Stresses

• Published in: Journal of plant biology = Singmul Hakhoe chi 2010, 53(2), , pp.142-149
• Main Authors: Liu, Ai-Ling, Hunan Agricultural University, Changsha City, Hunan Province, China, Zou, Jie, Hunan Agricultural University, Changsha City, Hunan Province, China, Zhang, Xian-Wen, Hunan Agricultural University, Changsha City, Hunan Province, China, Zhou, Xiao-Yun, Hunan Agricultural University, Changsha City, Hunan Province, China, Wang, Wen-Fang, Hunan Agricultural University, Changsha City, Hunan Province, China, Xiong, Xing-Yao, Hunan Agricultural University, Changsha City, Hunan Province, China, Chen, Li-Yun, Hunan Agricultural University, Changsha City, Hunan Province, China, Chen, Xin-Bo, Hunan Agricultural University, Changsha City, Hunan Province, China
• Format: Article
• Language: English
• Subjects: Abiotic stress; Aquatic plants; Biomedical and Life Sciences; Cold treatment; DNA polymerase; ESTRES; Expression profile; Gene expression; Genes; Heat; Heat shock; Heat shock factors; Heat shock transcription factor; Heat stress; Heat tolerance; Life Sciences; Original Research; ORYZA SATIVA; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Polyethylene glycol; Polymerase chain reaction; Rice; RNA-directed DNA polymerase; Seedlings; Seeds; STRESS; Temperature; Temperature effects; Transcription factors; 생물학
• ISSN: 1226-9239; 1867-0725
• DOI: 10.1007/s12374-010-9099-6

Expression profiles of 12 class A rice heat shock transcription factor genes (OsHsfAs) were analyzed by semi-quantitative reverse transcriptase polymerase chain reaction. The OsHsfA genes exhibited tissue-specific expressions under normal condition. OsHsfA1a, A2d, and A9 were predominantly expressed in young spike. Expression responses of the 12 OsHsfAs under abiotic stresses were analyzed in the shoots of rice seedling. Most OsHsfA genes responded quickly to heat stress except for OsHsfA1a, A3, and A9 which were almost unaffected. In particular, OsHsfA2a expression in response to heat stress was highest among the heat shock factors examined. However, the majority of the increased OsHsfAs expression responses to salt, polyethylene glycol (PEG), and cold treatments primarily occurred during the later stages (3 to 24 h) of stress exposure. Furthermore, most of OsHsfA gene expressions were little affected and only a few (OsHsfA3, A4d, A7, and A9) genes had slow responses to cold treatment. The results indicate that the transcript levels of OsHsfAs during heat stress exposure were distinct from those of plants subjected to salt, PEG, and cold stresses, suggesting that there might be different regulatory networks between heat and non-heat stress.