BIN2/DWF12 Antagonistically Transduces Brassinosteroid and Auxin Signals in the Roots of Arabidopsis

Plant growth-stimulating hormones brassinosteroids (BRs) function via interactions with other hormones. However, the mechanism of these interactions remains to be elucidated. The unique phenotypes of brassinosteroid insensitive2/dwarf12-D (bin2/dwf12-D) mutants, such as twisted inflorescences and le...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2011, 54(2), , pp.126-134
Main Authors: Maharjan, Puna Maya, Seoul National University, Seoul, Republic of Korea, Schulz, Burkhard, Purdue University, West Lafayette, IN, USA, Choe, S.H., Seoul National University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
ABA
Abscisic acid
AUXINAS
AUXINE
AUXINS
BIN2
Biomedical and Life Sciences
BRASINOSTEROIDES
BRASSINOSTEROIDE
BRASSINOSTEROIDS
Cell division
DWF4
DWF4 pro:GUS
Genes
Homeostasis
Hormones
Kinases
Lateral root
Life Sciences
Localization
Original Research
Phenotypes
Phosphorylation
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant growth
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Proteins
Root development
Roots
생물학
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Summary:Plant growth-stimulating hormones brassinosteroids (BRs) function via interactions with other hormones. However, the mechanism of these interactions remains to be elucidated. The unique phenotypes of brassinosteroid insensitive2/dwarf12-D (bin2/dwf12-D) mutants, such as twisted inflorescences and leaves, suggested that BIN2, a negative regulator of BR signaling, may be involved in auxin signaling. Furthermore, previously, we showed that auxin stimulates DWF4 expression. To determine the possible role of BIN2/DWF12 in Auxin signaling, we measured DWARF4pro:GUS activity through both GUS histochemical staining and in vivo GUS assay. We found that the GUS activity in the bin2/dwarf12-1D background dramatically increased relative to control. In addition, the number of lateral roots (LR) in bin2/dwf12-1D was greater than wild type, and the optimal concentration for auxin-mediated lateral root induction was lower in bin2/dwf12-1D; these findings suggest that BIN2 plays a positive role in auxin signaling. In contrast, ABA repressed both DWF4pro:GUS expression and lateral root development. However, the degree of repression was lower in bin2/dwf12-1D background, suggesting that BIN2 plays a role in ABA-mediated DWF4pro:GUS expression and subsequently in lateral root development, too. Therefore, it is likely that BIN2 plays a role of signal integrator for multiple hormones, such as BRs, auxin, and ABA.
ISSN:1226-9239
1867-0725
DOI:10.1007/s12374-010-9138-3