Characterization of a RAPD Fragment Unique to Species with Hairy Fruit Skin in the Genus Actinidia

To develop a SCAR primer related to the hairy-fruit trait in the genus Actinidia, we took a PCR-RAPD approach using arbitrary 10-mer primers. PCR with the UBC 376 primer generated specific fragments from three species with hairy fruit skin. Those fragments were then cloned to determine their nucleot...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2004, 47(3), , pp.210-215
Main Authors: Kim, Seong-Cheol, Jung, Yong-Hwan, Kim, Misun, Koh, Seok-Chan, Kwon, Jeong-Song, Kim, Ho-Bang
Format: Article
Language:Korean
Subjects:
Actinidia
cellulose synthase
RAPD
SCAR primer
생물학
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Summary:To develop a SCAR primer related to the hairy-fruit trait in the genus Actinidia, we took a PCR-RAPD approach using arbitrary 10-mer primers. PCR with the UBC 376 primer generated specific fragments from three species with hairy fruit skin. Those fragments were then cloned to determine their nucleotide sequences. Two SCAR primers were designed from the UBC 376 primer and nucleotide sequences were obtained from the PCR fragments. A SCAR primer, OKC385, specifically amplified a 385-bp fragment from one clone of Actinidia eriantha, four of Actinidia chinensis, and four of Actinidia deliciosa. Deduced amino acid sequences of this fragment showed high sequence homology with plant cellulose synthases, which are involved in the biosynthesis of cellulose, a major cell wall component. The 385-bp fragment was specifically detected only in the series Perfectae C.F. Liang of section Stellatae Li. This type has many hairs on the leaves, fruits, and stems, suggesting that the gene containing the PCR fragment is involved in hair formation in this phylogenetic group. Taken together, our results suggest that the SCAR primer, OKC385, can be used as a specific primer for early selection of the non-hair trait in breeding of the genus Actinidia.
ISSN:1226-9239
1867-0725