A Study of Cotyledon Senescence in Cucumber (Cucumis sativus L.) Based on Expressed Sequence Tags and Gene Expression

Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (＞95％ yel...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2004, 47(3), , pp.244-253
Main Author: Kim, Dae-Jae
Format: Article
Language:Korean
Subjects:
cotyledon
cucumber
EST
RT-PCR
senescence
생물학
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (＞95％ yellow) for studying the genes responsible for lipid mobilization during germination and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365 cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected for several known and unknown genes. These results were used to investigate the possible functions for senescence-related genes during cotyledon development.
ISSN:1226-9239
1867-0725